You Have Isolated DNA for PCR and Accidentally Added Topoisomerase

question 42

Multiple Choice

You have isolated DNA for PCR and accidentally added topoisomerase instead of polymerase to your sample. What would you expect if you ran this DNA sample on a gel?

• A)The DNA would show increased supercoiling and have a lower electrophoretic mobility.
• B)The DNA would show decreased supercoiling and have a higher electrophoretic mobility.
• C)The DNA would show increased supercoiling and have a higher electrophoretic mobility.
• D)The DNA would show decreased supercoiling and have a lower electrophoretic mobility.
• E)The DNA would show decreased supercoiling, but electrophoretic mobility will be unaffected.

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Definitions:

Overapplied Manufacturing Overhead

A situation where the allocated amount of manufacturing overhead costs exceeds the actual overhead costs incurred.

Unadjusted Cost of Goods Sold

The initial calculation of the cost of goods sold that has not been modified for returns, allowances, or discounts.

Overapplied Manufacturing Overhead

A scenario where the allocated manufacturing overhead costs exceed the actual overhead costs incurred, resulting in a credit balance.

Predetermined Overhead Rate

This is a rate used to allocate manufacturing overhead costs to individual units of production, based on a certain activity, such as labor hours or machine hours.