You Are Trying to Prepare a DNA Karyotype, but This

question 9

Multiple Choice

You are trying to prepare a DNA karyotype, but this time you forgot to add trypsin to your chromosome preparation. What effect would you expect to see?

• A)Dyes would stain the chromosomes darker because more proteins would be present to take up the dye.
• B)Dyes would not stain the chromosomes as expected because proteins would not be removed from the chromatin and so would block the dye from accessing the DNA.
• C)Chromosomes would not condense properly, so it would be very difficult to isolate individual chromosomes.
• D)Chromosomes would be degraded quickly because the trypsin is necessary to promote DNA stability by adding more scaffold proteins to the chromatin.
• E)The chromosomes would be more difficult to pair up because the trypsin acts as a dye, creating a banding pattern that distinguishes between chromosomes.

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Definitions:

Proximity

The physical closeness or distance between people, objects, or locations, often influencing communication, interaction, or relationships.

Decision Maker

A person or group responsible for making choices or judgments that influence the outcome of a situation or organization.

Self-Serving Principles

Cognitive biases or behaviors that lead individuals to see things in a way that benefits them personally, often at the expense of others or the truth.

Socially Acceptable

Behaviors or norms that are considered appropriate and acceptable within a society or social group.