Based only on this Southern blot and with no knowledge of which of the β-globin SNPs you are looking at, which lane(s)might represent(s)a heterozygous individual? 
Definitions:
Biological Components
Essential elements, structures, or molecules that are part of or necessary for the living organisms' life cycles and processes.
Phosphorus
A chemical element, essential for life, involved in many cellular processes, including energy transfer and the structure of DNA and RNA.
Inhalation
The act of drawing air or other substances into the lungs.
Primary Consumers
Organisms that feed directly on producers in a food chain, typically herbivores that eat plants.
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