We Can Detect the SNP in the β^S Allele Because

question 38

Essay

We can detect the SNP in the β^S allele because it destroys a DdeI restriction site, leading to DNA restriction fragment variation revealed by Southern blot analysis.
Either two or three restriction sequences for the restriction endonuclease DdeI can occur near the
β-globin gene, depending on the allele. One DdeI restriction sequence is eliminated from the β^S allele by the SNP in the mutant allele because of a mutation in the double-stranded target sequence 5′ - CTNAG - 3′.
Suppose the wild-type allele sequence is CCTGAG, while a newly identified SNP is CCTTAG. Would this same restriction fragment analysis technique be appropriate for detecting this particular SNP? What other technique(s)could you use to identify this particular mutation?

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Definitions:

Epigenetic Marks

Chemical modifications to DNA and histone proteins that affect gene expression without altering the underlying DNA sequence.

Environment

The surroundings or conditions in which a person, animal, or plant lives or operates, including both natural and human-made factors.

Natural Selection

The mechanism through which creatures that are more suited to their surroundings are more likely to live and have a greater number of descendants.

Genome

A genome is the complete set of DNA, including all of its genes, in an organism, encapsulating the entire genetic information.