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Before Transferring DNA from a Gel to the Membrane in Southern

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Essay

Before transferring DNA from a gel to the membrane in Southern blotting, the DNA must be denatured (usually by soaking the gel in NaOH). Why is this step necessary?


Definitions:

Lateral Edge

The edge that runs along the side of an object or structure.

Eyeball

The spherical part of the eye that contains the optic nerve, retina, and lens, responsible for vision.

Tears

A saline solution produced by the lacrimal glands in the eye, primarily used to lubricate the eyes and remove irritants.

Interrupted Transmission

A disruption in the flow of information or power from one point to another, often resulting in a loss of communication or failure of an electrical system.

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