We Can Detect the SNP in the β^S Allele Because

question 38

Essay

We can detect the SNP in the β^S allele because it destroys a DdeI restriction site, leading to DNA restriction fragment variation revealed by Southern blot analysis.
Either two or three restriction sequences for the restriction endonuclease DdeI can occur near the
β-globin gene, depending on the allele. One DdeI restriction sequence is eliminated from the β^S allele by the SNP in the mutant allele because of a mutation in the double-stranded target sequence 5′ - CTNAG - 3′.
Suppose the wild-type allele sequence is CCTGAG, while a newly identified SNP is CCTTAG. Would this same restriction fragment analysis technique be appropriate for detecting this particular SNP? What other technique(s)could you use to identify this particular mutation?

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