To Map Genes of a Bacterial Strain, Conjugation Must Be

question 26

Multiple Choice

To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a⁺b⁺c⁺d⁺e⁺str^R and F^- cells of genotype a^-b^-c^-d^-e^-str^S and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. What role does the blender play in the experiment?

• A)serves as a culture container only
• B)acts as a mechanism to bring cells into contact
• C)speeds up molecular movement of DNA
• D)shears the sex pili of conjugants
• E)hastens conjugation events

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