In Phi X 174 Phage, Whose Genome Is a Single-Stranded

question 45

Multiple Choice

In phi X 174 phage, whose genome is a single-stranded, circular DNA known as the - strand, replication occurs by the rolling circle method. In which order must the events of replication occur?

• A)nicking of the - strand, binding of relaxase protein to the 5′ end, DNA synthesis of a + strand, separation of + from - strands, circularization of both strands
• B)synthesis of a + strand, nicking at the origin, binding of protein to the 5′ end, DNA synthesis, displacement of the + strand when replication passes the origin, circularization of the + strand
• C)synthesis of a + strand, nicking of both strands to separate them, recircularization of both strands
• D)binding of a protein at the origin of the - strand, synthesis of a + strand, release of the + strand

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