To Map Genes of a Bacterial Strain, Conjugation Must Be

question 23

Multiple Choice

To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a⁺b+c+d+e+str^R and F^- cells of genotype a^-b-c-d-e-str^S and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. The resulting cultures were then plated on medium containing streptomycin. Why?

• A)to eliminate non-conjugating Hfr cells
• B)to eliminate all non-conjugated cells
• C)to eliminate non-conjugated F^- cells
• D)to selectively eliminate cells that have taken in F⁺ genes

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