Which Technique Can Be Used to Estimate the Relative Copy

question 75

Multiple Choice

Which technique can be used to estimate the relative copy numbers of particular DNA sequences in a sample?

• A) reverse transcriptase PCR
• B) quantitative PCR
• C) some gel based analysis such as with the Bioanalyzer and Fragment Analyzer gel band density are used in calculating concentration in a lot of labs.
• D) normal PCR
• E) in vitro transcription

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Definitions:

Vector DNA

Vector DNA refers to a molecule of DNA, such as a plasmid or virus, used to transfer genetic material into a cell in genetic engineering processes.

DNA Ligase

An enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

DNA Polymerase

An enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building blocks of DNA.

RNAi

Short for RNA interference, it is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules.