A Plasmid That Encodes Resistance to Ampicillin and Tetracycline Is

question 96

Short Answer

A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. (a) What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid? (b) What antibiotic-resistance phenotypes will be found on the plate? (c) Which phenotype will indicate the presence of plasmids that contain human DNA fragments?

---

Definitions:

Germinal Stage

The first fortnight of development before birth, which commences right after conception, is marked by quick proliferation of cells and the start of cellular specialization.

Psychologists

Professionals in the field of psychology, which involves the study of the mind and behavior.

Development

The process of growth and change that a person undergoes throughout their life, encompassing physical, cognitive, and emotional development.

Prenatal Development

The procedure of evolving and enlarging within the womb, starting at conception and concluding at birth.