Which Factor Is Required to Accurately Determine the Relative Amount

question 25

Multiple Choice

Which factor is required to accurately determine the relative amount of gene expression of two or more genes in an organism using qPCR?

• A) a "no template" control
• B) an oligonucleotide probe labeled with a fluorophore and a quenching molecule at its ends
• C) use of reverse transcriptase to copy the RNA products into DNA
• D) All of the answers are correct.
• E) None of the answers is correct.

---

Definitions:

Etiology

The study of the cause(s) of diseases and disorders.

Disorder

A disruption to normal functioning that can affect the body or mind and is often characterized by specific symptoms and signs.

Symptoms

Observable signs or indications of a disease, disorder, or condition.

Mental Illness

A wide range of mental health conditions that affect mood, thinking, and behavior, often resulting in distress or impairment in functioning.