What Is a Significant Problem with Regard to Making CDNA

question 45

Multiple Choice

What is a significant problem with regard to making cDNA libraries to analyze overall gene function in an eukaryotic organism?

• A) A cDNA library will only contain genes that are actively transcribed in the organism.
• B) Reverse transcriptase cannot be used to make cDNA libraries.
• C) cDNA cannot be amplified by PCR.
• D) cDNA libraries do not contain intron sequences.
• E) cDNA libraries may contain several alternatively spliced forms of the same gene.

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