A Scientist Wishes to Produce a Mammalian Protein in E

question 46

Essay

A scientist wishes to produce a mammalian protein in E. coli. The protein is a glycoprotein with a molecular weight of 40,000. Approximately 20% of its mass is polysaccharide. The isolated protein is usually phosphorylated and contains three disulfide bonds. The cloned gene contains no introns. (a) What sequences or sites will be required in the vector to get this gene regulated, transcribed, and translated in E. coli? (b) List two problems that might arise in producing a protein identical to that isolated from mammalian cells and describe each problem in no more than two sentences.

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Definitions:

Expert Power

Expert Power is the ability to influence others' attitudes or behaviors based on the perception of one's knowledge, expertise, or experience in a specific area.

Information Power

The influence or control over others that is gained through access to or possession of information.

Marketing Power

The ability of a company or brand to influence the buying behavior of consumers, shaping perceptions and driving demand for its products or services.

Cohesiveness

The degree to which members of a group or team are attracted to each other and motivated to stay in the group, contributing to its unity and success.