In DNA Sequencing by the Sanger (Dideoxy) Method

question 28

Multiple Choice

In DNA sequencing by the Sanger (dideoxy) method:

• A) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesis of complementary strands.
• B) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are then separated by electrophoresis.
• C) the dideoxynucleotides must be present at high levels to obtain long stretches of DNA sequence.
• D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.
• E) the template DNA strand is radioactive.

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Definitions:

Carbon Atoms

The fundamental building blocks of organic chemistry, with the atomic number 6, capable of forming a vast array of compounds, polymers, and structures.

Hydrogen Atoms

The simplest type of atom, consisting of one proton and one electron, fundamental in the structure of all organic compounds.

Molar Heat

The amount of heat absorbed or released by one mole of a substance during a phase change, without a change in temperature.

Combustion

A chemical process involving the rapid oxidation of substances, resulting in the release of heat, light, and products like CO2 and water.