In the Chemical Synthesis of DNA

question 74

Multiple Choice

In the chemical synthesis of DNA:

• A) the dimethoxytrityl (DMT) group catalyzes formation of the phosphodiester bond.
• B) the direction of synthesis is 5' to 3'.
• C) the maximum length of oligonucleotide that can be synthesized is 8-10 nucleotides.
• D) the nucleotide initially attached to the silica gel support will become the 3' end of the finished product.
• E) the protecting cyanoethyl groups are removed after each step.

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