What Would Happen If Blocking Tags Were NOT Used in Reversible

question 40

Multiple Choice

What would happen if blocking tags were NOT used in reversible terminator sequencing, a next-generation method of DNA sequencing?

• A) The DNA fragments to sequence/read would be too large.
• B) More than one dNTP could be incorporated to the same growing nucleotide chain during a single cycle.
• C) The flow cell could not immobilize DNA fragments.
• D) Multiple fluorescent colors could be observed across the flow cell at the same time.
• E) Luciferase would not react in the presence of ATP.

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