An Enzyme Catalyzes the Reaction A $\rightarrow$ B the Initial Rate of the Reaction Was Measured as Rate

question 104

Short Answer

An enzyme catalyzes the reaction A $\rightarrow$ B. The initial rate of the reaction was measured as a function of the concentration of A. The following data were obtained:
[A], micromolar
V₀, nmoles/min
0.05
0.08
0.1
0.16
0.5
0.79
1
1.6
5
7.3
10
13
50
40
100
53
500
73
1,000
76
5,000
79
10,000
80
20,000
80
a) What is the K_m of the enzyme for the substrate A?
b) What is the value of V₀ when [A] = 43?
The above data were plotted as 1/V₀ versus 1/[A], and a straight line was obtained.
c) What is the value of the y-intercept of the line?
d) What is the value of the x-intercept of the line?

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