You Measure the Initial Rate of an Enzyme Reaction as a Function

question 22

Essay

You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained:
[S]
V₀
-Inhibitor
+Inhibitor
0.0001
33
17
0.0002
50
29
0.0005
71
50
0.001
83
67
0.002
91
80
0.005
96
91
0.01
98
95
0.02
99
98
0.05
100
99
0.1
100
100
0.2
100
100
a) What is the V_max in the absence of inhibitor?
b) What is the K_m in the absence of inhibitor?
c) When [S] = 0.0004, what will V₀ be in the absence of inhibitor?
d) When [S] = 0.0004, what will V₀ be in the presence of inhibitor?
e) What kind of inhibitor is it likely to be?

Evaluate the implications of excess capacity in monopolistically competitive firms.

Analyze the impact of government interventions on firm output and efficiency in monopolistically competitive markets.

Understand the role of product differentiation in shaping monopolistically competitive market structures.

Analyze the determinants of the equilibrium price and quantity in monopolistically competitive markets.

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Definitions:

Value Stream Mapping

A lean management tool for analyzing the flow of materials and information currently required to bring a product or service to a consumer.

Time Function Mapping

A visual representation technique used in process improvement to analyze the flow of information or material through a process over time.

Supply Chain

The network of organizations involved in producing, delivering, and selling goods and services, from the acquisition of raw materials to the final consumer.

Crossover Point

The point at which two different options have equal costs or benefits, influencing a change in decision.