The Double-Reciprocal Transformation of the Michaelis-Menten Equation, Also Called the Lineweaver-Burk

question 28

Multiple Choice

The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by 1/V₀ = K_m /(V_max[S]) + 1/V_max
To determine K_m from a double-reciprocal plot, you would:

• A) multiply the reciprocal of the x-axis intercept by -1.
• B) multiply the reciprocal of the y-axis intercept by -1.
• C) take the reciprocal of the x-axis intercept.
• D) take the reciprocal of the y-axis intercept.
• E) take the x-axis intercept, where V₀ = 1/2 V_max.

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