You Want to Purify a Specific Soluble Protein from a Solution

question 9

Multiple Choice

You want to purify a specific soluble protein from a solution of lysed cells so that you can conduct biochemical tests of the protein's function. To best achieve this, which type of antibody would be MOST effective to use in your affinity purification?

• A) monoclonal IgE
• B) polyclonal IgE
• C) monoclonal IgG
• D) polyclonal IgG
• E) monoclonal IgM

---

Definitions:

Methane Groups

Collections of organisms, typically microbes, involved in the production or consumption of methane, a potent greenhouse gas.

Male Pipefishes

Marine fish species in which males are responsible for pregnancy and birth, showcasing a unique reversal of traditional reproductive roles.

Offspring Investment

The amount of resources (time, energy, food) an organism expends on the care and development of its young.

Mother Hypothesis

A theory suggesting that certain behaviors or traits in females, including those influencing fertility and offspring survival, have evolved due to maternal investment and efforts to ensure the propagation of genes.