Describe the Process of Using ELISA to Detect the Presence

question 67

Essay

Describe the process of using ELISA to detect the presence of anti-HIV protease IgG antibodies in human blood samples. Include how the assay would be prepared, the order of reagent addition, and how differing levels of anti-HIV protease antibodies could be detected by the assay.

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Definitions:

Ocular Lens

The lens within a microscope or camera that is closest to the eye when someone looks through the device.

Objective Lens

The lens in a microscope that is closest to the specimen, magnifying the image.

Mechanical Stage Control

A feature on microscopes that allows for precise movement of the specimen slide in the horizontal plane, facilitating detailed examination.

Fine Adjustment Knob

A part of a microscope used to make small adjustments in focus, enhancing the clarity of the image.