The Image Below Plots the Denaturation of Ribonuclease a as a Function

question 58

Multiple Choice

The image below plots the denaturation of ribonuclease A as a function of guanidine hydrochloride (GdnHCl) concentration, monitored by circular dichroism. The shape of the curve in the figure supports which statement?

• A) The addition of GdnHCl has little effect on protein secondary structure.
• B) Increasing temperature causes the protein to become unfolded.
• C) Unfolding of this protein is a cooperative process.
• D) Ribonuclease A is a heat-stable protein.
• E) The peptide bonds of the protein are broken around 3.4 M GdnHCl.

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