A Biochemist Is Attempting to Separate a DNA-Binding Protein (Protein

question 5

Short Answer

A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in a solution. Only three other proteins (A, B, and
C) are present. The proteins have the following properties:
pI
(isoelectric
Size
Bind to
point)
M_r
DNA?
protein A
7.4
82,000
yes
protein B
3.8
21,500
yes
protein C
7.9
23,000
no
protein X
7.8
22,000
yes
What type of protein separation techniques might she use to separate:
(a) protein X from protein A?
(b) protein X from protein B?
(c) protein X from protein C?

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