To Determine the Isoelectric Point of a Protein, First Establish

question 66

Multiple Choice

To determine the isoelectric point of a protein, first establish that a gel:

• A) contains a denaturing detergent that can distribute uniform negative charges over the protein's surface.
• B) exhibits a stable pH gradient when ampholytes become distributed in an electric field.
• C) is washed with an antibody specific to the protein of interest.
• D) neutralizes all ionic groups on a protein by titrating them with strong bases.
• E) relates the unknown protein to a series of protein markers with known molecular weights, M_r.

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