The First Step in Two-Dimensional Gel Electrophoresis Generates a Series

question 41

Multiple Choice

The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. In a second step, a strip of this gel is turned 90 degrees, placed on another gel containing SDS, and electric current is again applied. In this second step:

• A) proteins with similar isoelectric points become further separated according to their molecular weights.
• B) the individual bands become stained so that the isoelectric focus pattern can be visualized.
• C) the individual bands become visualized by interacting with protein-specific antibodies in the second gel.
• D) the individual bands undergo a second, more intense isoelectric focusing.
• E) the proteins in the bands separate more completely because the second electric current is in the opposite polarity to the first current.

Analyze the significance and use of aptitude and mechanical tests in career counseling.

Appreciate the role of online databases and career exploration tools in career planning.

Understand the importance of assessing job satisfaction predictors through interest inventories.

Learn about the components and scoring of multi-dimensional career assessment tools.

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Definitions:

Empathetic and Compassionate

Qualities of understanding and sharing the feelings of others, while also having a desire to help and alleviate their distress.

Future Job Behavior

Predicted actions or performance of an individual in a job based on their past behavior or other indicators.

Criterion-related Validation

A method of assessment where a test is evaluated based on its ability to predict an outcome or whether it correlates with a specific criterion.

Validity Generalization

A statistical approach that concludes whether the validity of a test or selection procedure can be generalized across different contexts or groups.