One Method Used to Prevent Disulfide Bond Interference with Protein

question 81

Multiple Choice

One method used to prevent disulfide bond interference with protein sequencing procedures is:

• A) cleaving proteins with proteases that specifically recognize disulfide bonds.
• B) protecting the disulfide bridge against spontaneous reduction to cysteinyl sulfhydryl groups.
• C) reducing disulfide bridges and preventing their re-formation by further modifying the -SH groups.
• D) removing cystines from protein sequences by proteolytic cleavage.
• E) sequencing proteins that do not contain cysteinyl residues.

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