A Biochemist Wishes to Determine the Sequence of a Protein

question 52

Essay

A biochemist wishes to determine the sequence of a protein that contains 123 amino acid residues. After breaking all of the disulfide bonds, the protein is treated with cyanogen bromide (CNBr), and it is determined that that this treatment breaks up the protein into seven conveniently sized peptides, which are separated from each other. It is your turn to take over. Outline the steps you would take to determine, unambiguously, the sequence of amino acid residues in the original protein.

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Definitions:

Unpleasant Experiences

Experiences that cause discomfort, distress, or pain, either physically or emotionally.

Elaborative

Pertaining to providing additional information or detail that enhances understanding, often used in memory strategies or learning techniques.

Repetitive

Characterized by repeating the same task or action over and over again.

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Relating to or effective in the future; often used to describe studies or methods that observe outcomes over a certain period.