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Cyanide ion (CN-) blocks electron transfer in mitochondria at the level of cytochrome a + a3. 2,4-Dinitrophenol (DNP) is an uncoupler of mitochondrial oxidative phosphorylation. Draw graphs (ATP synthesized vs. time and O2 consumed vs. time) with labeled curves to show the effects of adding each of these compounds separately to a suspension of mitochondria supplied with O2, succinate, ADP, and Pi. Use an arrow to indicate the time of drug addition.
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