Lactate Dehydrogenase Catalyzes the Reversible Reaction:
Pyruvate + NADH $\rightarrow$ Lactate + NAD⁺
Given the Following Facts (A) Tell in H⁺

question 70

Short Answer

Lactate dehydrogenase catalyzes the reversible reaction:
pyruvate + NADH + H⁺ $\rightarrow$ lactate + NAD⁺
Given the following facts (a) tell in which direction the reaction will tend to go if NAD⁺, NADH, pyruvate, and lactate were mixed, all at 1 M concentrations, in the presence of lactate dehydrogenase at pH 7; (b) calculate $\Delta$ G'° for this reaction. Show your work.
NAD⁺ + H⁺ + 2e^- $\rightarrow$ NADH
E'° = -0.32 V
pyruvate + 2H⁺ + 2e^- $\rightarrow$ lactate
E'° = -0.19 V
The Faraday constant, , is 96.48 kJ/V · mol.

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