You Are Trying to Isolate an Enzyme That Catalyzes the Conversion

question 83

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You are trying to isolate an enzyme that catalyzes the conversion of A $\rightarrow$ B, and you have a sensitive assay for this enzyme. After lysing open the cells, you find that the activity is associated with the membrane fraction, not the soluble fraction (which you throw away). You then follow the procedure shown below. Explain the sudden re-occurrence of activity at the end of your protocol.

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Zero-balance Account

A banking arrangement where a company’s bank account is maintained at a zero balance, with funds being transferred from a master account as needed.

Disbursement Purpose

The specific reason or objective for which funds are paid out or disbursed in a financial transaction.

Cheques Presented

Refers to the cheques that have been submitted to a bank for payment and are pending clearance.

Money Market Account

A type of savings account that typically offers higher interest rates, with some restrictions on withdrawal.