Reagents a and B Both React Covalently with Primary Amino

question 44

Essay

Reagents A and B both react covalently with primary amino groups such as those of phosphatidylethanolamine. Reagent A permeates erythrocytes, but reagent B is impermeant. Both A and B are available in radioisotopically labeled form. Describe a simple experiment by which you might determine whether the phosphatidylethanolamine of erythrocyte membranes is located in the outside face of the lipid bilayer, the inside face, or in both.

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Definitions:

Stressor

Any event, experience, or environmental stimulus that causes stress in an individual.

Experimental Group

In an experiment, this group receives or is exposed to the treatment or condition being tested, in contrast to the control group.

Independent Variable

In an experimental setting, the variable that is manipulated or controlled by the researcher to observe its effects on the dependent variable.

Control Group

In experimental research, a group of participants that does not receive the experimental treatment, serving as a comparison to evaluate the effect of the treatment.