You Are Trying to Isolate an Enzyme That Catalyzes the Conversion

question 83

Not Answered

You are trying to isolate an enzyme that catalyzes the conversion of A $\rightarrow$ B, and you have a sensitive assay for this enzyme. After lysing open the cells, you find that the activity is associated with the membrane fraction, not the soluble fraction (which you throw away). You then follow the procedure shown below. Explain the sudden re-occurrence of activity at the end of your protocol. $You are trying to isolate an enzyme that catalyzes the conversion of A \rightarrow B, and you have a sensitive assay for this enzyme. After lysing open the cells, you find that the activity is associated with the membrane fraction, not the soluble fraction (which you throw away). You then follow the procedure shown below. Explain the sudden re-occurrence of activity at the end of your protocol.$

Definitions:

Liability

The state of being legally responsible for something, particularly in terms of debts or legal obligations.

Partnership

An authorized business arrangement where two or more partners share both the administration and the profits.

Dissolution

The closure or termination of an entity's existence, such as a corporation or partnership.

UPA

Uniform Partnership Act, which is legislation adopted by various states in the USA to govern partnership formation and operation.