Why Is a "No DNA" Control Used When Performing Molecular

question 10

Multiple Choice

Why is a "no DNA" control used when performing molecular tests using the polymerase chain reaction (PCR) ?

• A) To ensure that the targeted DNA is amplified
• B) To ensure that nontargeted DNA is negative
• C) To ensure that the targeted DNA containing the mutation is positive
• D) To ensure that no DNA cross-contamination of the samples, controls, or reagents occurs

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Definitions:

Reaction

The process by which chemical substances transform into different substances, often involving the breaking and forming of bonds between atoms.

Reaction

A process in which substances interact to form new substances with different physical and chemical properties.

Pentanamide

An organic compound characterized by the presence of a five-carbon chain terminated with an amide group, with the formula CH3(CH2)3CONH2.

1-Pentanamine

An organic compound, a type of primary amine with the chemical formula C5H13N, used in chemical synthesis and as an intermediate.