Imagine You Wanted to Use a Human Genomic DNA Library

question 25

Multiple Choice

Imagine you wanted to use a human genomic DNA library to clone the human gene for insulin. You will be using the rat insulin gene sequence as your DNA probe. In what order would you perform the following steps to accomplish this goal? 1. Use autoradiography to identify colonies containing DNA that hybridized to the probe
2) Grow transformed cells on media with antibiotics and X-gal for blue-white screening
3) Ligate genomic DNA and vector DNA
4) Cut genomic DNA and vector DNA with restriction enzymes
5) Hybridize library DNA with labeled probe for the rat insulin gene
6) Transform bacteria with recombinant plasmid

• A) 2, 3, 6, 5, 4, 1
• B) 1, 2, 5, 6, 4, 3
• C) 4, 3, 6, 2, 5, 1
• D) 4, 3, 5, 2, 6, 1
• E) 2, 6, 5, 3, 1, 4

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Definitions:

Buffer

A temporary storage area used to manage data while it is being transferred from one place to another, ensuring smooth processing.

Neutral

Not supporting or favoring either side in a conflict, competition, or dispute.

Noncontroversial

Describing topics or issues that are unlikely to provoke disagreement or debate.

Indirect Message

Communication that conveys a point in a roundabout or less straightforward manner, often used to soften the delivery of sensitive information.