Explain the Dideoxy Sequencing Method

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The Answer is Sanger's method, which is also referred to as chain termination, is based on the use of dideoxynucleotides (ddNTP's) in addition to the normal nucleotides (NTP's) found in DNA. Dideoxynucleotides are essentially the same as nucleotides except they contain a hydrogen group on the 3' carbon instead of a hydroxyl group (OH). These modified nucleotides, when integrated into a sequence, prevent the addition of further nucleotides. This occurs because a phosphodiester bond cannot form between the dideoxynucleotide and the next incoming nucleotide, and thus the DNA chain is terminated. A nested set of products can then be used to determine the actual nucleotide sequence.

Explain the Dideoxy Sequencing Method

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