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Translation in a Test Tube Does Not Require an AUG

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Translation in a test tube does not require an AUG codon to start translation, but can start anywhere. Once translation begins, however, it translates successive triplets just like translation in a cell. Consult the standard genetic code and specify what polypeptide sequence (or sequences) you would expect to be translated from an mRNA molecule with the sequence 5'-CAGCAGCAGCAG … -3' if translation occurred in a test tube.


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