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In Order to Analyze How Different Components of the Lac

question 45

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In order to analyze how different components of the lac operon work, scientists created special strains of E. coli, called partial diploids. A partial diploid has one full copy of the lac operon in the bacterial chromosome plus another copy of the lac operon in a plasmid. Hence, for the lac operon (and only the lac operon) the bacterial cell is a diploid. Below is one possible genotype of a partial diploid. The genotype written to the left of the slash (/) is that of the lac operon in the bacterial chromosome, and the genotype written to the right of the slash is that of the lac operon in the plasmid. I+ P+ O+ Z-/I+ P+ O+ Z+
For the partial diploid genotype shown here, determine whether functional -galactosidase is synthesized in the absence and in the presence of lactose (and the accompanying allolactose that is the actual inducer) .


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