In the Diagram Below, the Horizontal Lines Represent DNA Strands

question 129

Multiple Choice

In the diagram below, the horizontal lines represent DNA strands in a double-stranded molecule, the vertical lines mark the positions of cleavage sites for a particular restriction enzyme, the arrows show the positions of primers used in the polymerase chain reaction (PCR) to amplify the region, and the numbers are the number of nucleotides between successive restriction sites. If this particular DNA molecule is amplified and then cleaved with the restriction enzymes, what size band(s) would be observed in a gel?

• A)5 kb
• B)5 kb and 3 kb
• C)5 kb, 3 kb, and 2 kb
• D)3 kb and 2 kb
• E)None of the answer options is correct.

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