You Have a Sequence of DNA with a Site on Which

question 163

Multiple Choice

You have a sequence of DNA with a site on which the restriction enzyme EcoRI will act. EcoRI recognizes the sequence below and cuts each strand at the locations indicated by the arrows. Below is your sequence of DNA: $5^{\prime}-$ ATTCGCGGAATTCTGG-3'
$3^{\prime}-$ TAAGCGCCTTAAGACC-5' You cut the sequence with EcoRI. What fragments are produced?

• A) \(\begin{array}{lr} 5^{\prime}-\mathrm{ATTCGCGG}-3{ }^{\prime} & 5^{\prime}-\mathrm{AATTCTGG}-3^{\prime} \\ 3^{\prime}-\mathrm{TAAGCGCCTTAA}-5^{\prime} & 3^{\prime}-\mathrm{GACC}-5^{\prime} \end{array}\)
• B) \(\begin{array}{lr} 5^{\prime}-\mathrm{ATTCGCGGAATT}-3^{\prime} & 5^{\prime}-\mathrm{CTGG}-3 ' \\ 3^{\prime}-\mathrm{TAAGCGCC}-5^{\prime} & 3^{\prime}-\mathrm{TTAAGACC}-5^{\prime} \end{array}\)
• C) \(\begin{array}{ll} 5^{\prime}-\mathrm{ATTCGCGG}-3^{\prime} & 5^{\prime}-\mathrm{AATTCTGG}-3^{\prime} \\ 3^{\prime}-\mathrm{TAAGCGCC}-5^{\prime} & 3^{\prime}-\mathrm{TTAAGACC}-5^{\prime} \end{array}\)
• D) \(\begin{array}{lr} 5^{\prime}-\mathrm{ATTCGCG}-3^{\prime} & 5^{\prime}-\mathrm{GAATTCTGG}-3^{\prime} \\ 3^{\prime}-\mathrm{TAAGCGCCTTAAG}-5^{\prime} & 3^{\prime}-\mathrm{ACC}-5^{\prime} \end{array}\)
• E) \(\begin{array}{lr} 5^{\prime}-\mathrm{ATTCGCGG}-3^{\prime}&5^{\prime}-\mathrm{AATT}-3^{\prime}&5^{\prime}-\mathrm{CTGG}-3^{\prime}\\3^{\prime}-\mathrm{TAAGCGCC}-5^{\prime}&3^{\prime}-\mathrm{TTAA}-5^{\prime}&3^{\prime}-\mathrm{GACC}-5^{\prime} \end{array}\)

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