A Shotgun Genome Sequencing Project Is Designed in Which the Average

question 57

Multiple Choice

A shotgun genome sequencing project is designed in which the average length of a sequenced fragment is 150 nucleotides and each nucleotide is to be sequenced an average of 20 times. For a genome that is 3 × 10⁷ base pairs long, how many fragments of 150 nucleotides would need to be sequenced?

• A)(3 × 10⁷)× 20 × 150 = 9 × 10¹⁰
• B)(3 × 10⁷)× 150 ÷ 20 = 2.25 × 10⁸
• C)(3 × 10⁷)÷ (20 × 150)= 1 × 10⁴
• D)(3 × 10⁷)× 20 ÷ 150 = 4 × 10⁶

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