Whole Genome Sequencing Is Often Approached by a Shotgun Sequencing

question 186

Multiple Choice

Whole genome sequencing is often approached by a shotgun sequencing technique in which large genomes are:

• A)randomly digested, short fragments are sequenced, and the overlapping sequences are assembled in order.
• B)digested in a few specific spots, large fragments are sequenced, and the overlapping sequences are assembled in order.
• C)randomly digested, short fragments are sequenced, and the overlapping sequences are assembled from largest to smallest.
• D)randomly digested, short fragments are sequenced, and the overlapping sequences are assembled from smallest to largest.
• E)All of these choices are correct.

Understand how body water content varies among individuals and comprehend factors influencing these variations.

Identify predominant ions in extracellular and intracellular fluids.

Recognize the role and regulation of electrolytes in physiological processes.

Understand the impact of hormones on electrolyte balance and fluid homeostasis.

---

Definitions:

Pathogen-Associated Molecules

Molecular structures that are recognized by the cells of the innate immune system, indicating the presence of microbial pathogens.

Cytokines

Small proteins released by cells that have a specific effect on the interactions between cells, on communications between cells or on the behavior of cells.

Cell Walls

Rigid or semi-rigid structures that surround the cell membrane of some cells, providing structural support and protection.

T Cytotoxic Cell

A type of T lymphocyte that kills cancer cells, cells that are infected (particularly with viruses), or cells that are damaged in other ways.