The Oligonucleotide Primers Used in the Polymerase Chain Reaction Are $\cdots$

question 151

Multiple Choice

The oligonucleotide primers used in the polymerase chain reaction are typically 20-30 nucleotides in length or longer; however, for purposes of this problem let's assume that six nucleotides is long enough. You wish to amplify the fragment shown below (the raised dots indicate several kilobases of DNA sequence not shown) and decide to design primers corresponding to the regions that are shown in bold. What primer sequences would you use? 5 '-TCAAACTTGCATGTG $\cdots$ GAGCCATGTGGCACAAA-3'
3'-AGTTTGAACGTACAG $\cdots$ CTCGGTACACCGTGTTT-5 '

• A)5'-GCAAGT-3' and 5'-TGCCAC-3'
• B)5'-ACTTGC-3' and 5'-GTGGCA-3'
• C)5'-GCAAGT-3' and 5'-GTGGCA-3'
• D)5'-ACTTGC-3' and 5'-TGCCAC-3'

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Definitions:

Bandwidth

Volume or capacity of a data transmission medium; the number of packets that can be pumped down a channel or multiplex backbone.

Volume Of Data

Describes the quantity of data, often measured in bytes, kilobytes, megabytes, gigabytes, terabytes, etc., that is created, stored, processed, and transferred.

J1939 Data Backbones

A type of network protocol used in heavy-duty vehicles for communication among various vehicle systems, based on CAN (Controller Area Network) technology.

Voltage Differential

The difference in electrical potential between two points, important for understanding the flow of electric current in circuits.