A Molecular Biologist Carries Out a PCR Reaction and Discovers

question 123

Essay

A molecular biologist carries out a PCR reaction and discovers to his disappointment that no PCR amplification takes place. It turns out that the problem is one of the primers, which has the following sequence:

5'-ATAACACTAAATAGCGCTATTTAGTGTTAT-3'

Can you suggest a reason why this primer does not work for PCR?

---

Definitions:

Operating Activities

Activities directly related to the day-to-day operations of a business, including sales, provision of services, and costs.

Cash Position

An indicator of a company's liquidity, showing the amount of cash or cash equivalents it has on hand at a given time.

Balance Sheet

A financial statement that provides a snapshot of a company's financial condition at a single point in time by detailing assets, liabilities, and equity.

Operating Activities

The day-to-day actions of a business involved in producing and selling its goods or services, which are reflected in the cash flow from operating activities.