A Messenger RNA from a Human Gene That Is 4

question 50

Multiple Choice

A messenger RNA from a human gene that is 4 kb in length is used as a probe in a Southern blot to identify restriction fragments of chromosomal DNA to which the mRNA can hybridize. Restriction fragments of DNA of 4 kb and 5 kb are observed to hybridize with the mRNA probe. What could explain how a 4 kb probe can hybridize with fragments that together sum to 9 kb?

• A)The gene from which the mRNA probe is transcribed may contain one or more introns.
• B)A restriction fragment may extend beyond the 5' end of the transcription start site.
• C)A restriction fragment may extend beyond the 3' end of the transcription stop site.
• D)The gene from which the mRNA probe is transcribed may contain introns, or the restriction fragments may extend beyond the 5' or 3' ends of the transcription stop site.
• E)The result must be some sort of mistake.

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