Using PCR, a Student Hopes to Amplify a Sequence of Known

question 67

Multiple Choice

Using PCR, a student hopes to amplify a sequence of known DNA from a genomic DNA sample. The two primers are both designed to pair with sequences that have a 40% GC content. Using a particular set of denaturation temperatures, annealing temperatures, and extension temperatures, the experiment produces not the expected single DNA fragment of a single known size, but a set of fragments of different sizes. Which of the following could account for these results?

• A)The sequences of the primers were not specific enough.
• B)The annealing temperature was too high.
• C)The denaturation temperature was too high.
• D)The extension temperature was too low.

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