You Are Interested in Studying Human ß-Globin and Decide to Produce

question 59

Multiple Choice

You are interested in studying human ß-globin and decide to produce the protein in bacterial cells. The ß-globin gene is a relatively simple gene with a single intron. You insert the entire gene, including the gene's promoter, in a plasmid and transform the recombinant plasmid into E. coli. You are disappointed to discover that your bacterial culture does not produce the correct protein. What are some possible explanations?

• A)The bacterial cell is missing RNA polymerase.
• B)The bacterial cell cannot splice introns.
• C)The bacterial cell does not recognize the eukaryote promoter.
• D)The bacterial cell produces the protein but it is rapidly degraded.

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Definitions:

Conjunctiva

A thin, clear membrane lining the eyelid and covering the white part of the eyeball, responsible for keeping the eye moist.

Dryness

A condition characterized by a lack of moisture or water.

Emmetropia

A state of normal vision where the eyes can focus images directly on the retina, resulting in clear vision at all distances.

Normal Vision

The ability to see without impairment that fits within the standard visual acuity range, typically measured as 20/20 vision.