A Certain Restriction Enzyme Cleaves Double-Stranded DNA at the Sequence $\begin{array}{l}\text { 5'-AAA/TTT-3' } \\\text { 3'-TTT/AAA-5' }\end{array}$

question 74

Multiple Choice

A certain restriction enzyme cleaves double-stranded DNA at the sequence shown below, where the slash indicates where each strand is cleaved. Note that the cleavage results in a two-base pair single-stranded region at the 5' end that allows the cleaved ends to undergo base pairing. 5'-AA/ATTT-3'
3'-TTTA/AA-5'
Which of the restriction enzymes, that cleave double-stranded DNA as shown below, would produce overhanging ends able to pair with those produced by the enzyme described above?

• A)
• \(\begin{array}{l}
• \text { 5'-AAA/TTT-3' } \\
• \text { 3'-TTT/AAA-5' }
• \end{array}\)
• B)
• \(\begin{array}{l}
• 5^{\prime} \text {-AATA/TT-3' } \\
• \text { 3'-TT/ATAA-5' }
• \end{array}\)
• C)
• \(\begin{array}{l}
• \text { 5'-GG/ATCC-3' } \\
• \text { 3'-CCTA/GG-5' }
• \end{array}\)
• D)
• \(\begin{array}{l}
• 5^{\prime}-\mathrm{GGA} / \mathrm{TCC}-3^{\prime} \\
• 3^{\prime}-\mathrm{CCT} / \mathrm{AGG}-5 ^{\prime}
• \end{array}\)
• E)
• \(\begin{array}{l}
• 5^{\prime}-\mathrm{GGAT} / \mathrm{CC}-3^{\prime} \\
• 3^{\prime}-\mathrm{CCTA} / \mathrm{GG}-5^{\prime}
• \end{array}\)

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