A Graduate Student Wants to Create a Recombinant DNA Molecule

question 90

Multiple Choice

A graduate student wants to create a recombinant DNA molecule and introduce this molecule into bacteria. What is the CORRECT order of steps that he should follow?

• A)choose plasmid/donor DNA  cut with restriction enzymes  join fragments via DNA ligase  transform bacteria
• B)choose plasmid  cut with restriction enzymes  join fragments with DNA ligase  introduce donor DNA  transform bacteria
• C)join plasmid and donor DNA using DNA ligase  cut with restriction enzymes  transform bacteria
• D)transform bacteria  choose plasmid/donor DNA  join fragments with DNA ligase
• E)cut plasmid/donor DNA with restriction enzymes  transform bacteria  isolate donor DNA/plasmid  join fragments with DNA ligase

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Definitions:

Secured Bonds

Bonds that are backed by collateral, such as real estate or other assets, to reduce the risk of default to investors.

Bond Principal

The face value of a bond, or the amount of money the bond issuer will pay back to the bondholder at maturity.

Serial Bonds

Bonds that are issued under the same contract but mature at different times, allowing the issuer to spread the repayment over several periods.

Principal Retirement

The act of paying off the original sum of money borrowed in a loan, excluding interest payments.