Using PCR, a Student Wants to Amplify a Sequence of Known

question 158

Multiple Choice

Using PCR, a student wants to amplify a sequence of known DNA from a genomic DNA sample. The two primers are both designed to pair with sequences that have a 40% GC content. Using a particular set of denaturation temperatures, annealing temperatures, and extension temperatures, the experiment does not produce the expected single DNA fragment of a single known size, but instead a set of fragments of varied sizes. Which of the following could account for these results?

• A)The annealing temperature was too low so that the primers can bind with multiple genomic sequences that contain some mismatched bases.
• B)The denaturation temperature was too high so that the primers can bind with each other as well as with multiple genomic sites containing mismatches.
• C)The extension temperature was too low so that many fragments were terminated before the full fragment was replicated.

Understand the impact of aging on society and individual identity.

Recognize the processes and effects of resocialization in distinct settings.

Understand the fundamental principles of negotiable instruments.

Differentiate between the roles and obligations of parties involved in negotiable instruments.

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Definitions:

Classical Conditioning

An associative learning process in which two stimuli are paired repeatedly, causing a response that initially comes from the second stimulus to eventually come from the first stimulus alone.

Operant Conditioning

A learning process by which the strength of a behavior is modified by reinforcement or punishment.

Unconditioned Response

A natural, automatic reaction to a stimulus that occurs without any need for prior learning or conditioning.

Conditioned Response

is a learned reaction to a previously neutral stimulus that has become associated with an unconditioned stimulus through conditioning.